targeted delivery of 5-fluorouracil with monoclonal antibody modified bovine serum albumin nanoparticles

Authors

ghazal fadaeian biotechnology group, chemical engineering faculty, tarbiat modares university, tehran, iran

seyed abbas shojaosadati biotechnology group, chemical engineering faculty, tarbiat modares university, tehran, iran

hasan kouchakzadeh biotechnology group, chemical engineering faculty, tarbiat modares university, tehran, iran

fazel shokri department of immunology, school of public health, tehran university of medical science, tehran, iran

abstract

herein, 1f2, an anti-her2 monoclonal antibody (mab), was covalently coupled to the surface of 5-fluorouracil (5-fu) loaded bovine serum albumin (bsa) nanoparticles. concerning two different crosslinkers for conjugation of 1f2, maleimide-poly (ethylene glycol)-succinimidyl carbonate (mal-peg5000-nhs) was selected due to its higher conjugation efficiency (23±4 %) obtained in comparison to n-succinimidyl 3-(2-pyridyl dithio) propionate (spdp) (8±2 %). a slight increase in the average particle size with a negligible prolongation of the 5-fu release was observed after 1f2 coupling. the 1f2-coupled 5-fu-loaded bsa nanoparticles interacted with nearly all her2 receptors available on the surface of her2-positive skbr3 cells. no cellular uptake was observed for her2-negative mcf7 cells. physicochemical and biological properties of the mab-modified nanoparticles did not significantly alter after three months of storage at room temperature. the in vitro cytotoxicity evaluation by mtt assay, demonstrated lower skbr3 viability (50.7±9 %) after 5 hours contact with 1f2-coupled 5-fu-loaded bsa nanoparticles in comparison with the other control systems due to their cell attachment and internalization after washing. in addition, no significant toxicity was observed on mcf7 cells. this novel system can efficiently be employed for targeted delivery of 5-fu to her2-positive cancerous cells.

Upgrade to premium to download articles

Already have an account?login

similar resources

Targeted Delivery of 5-fluorouracil with Monoclonal Antibody Modified Bovine Serum Albumin Nanoparticles

Herein, 1F2, an anti-HER2 monoclonal antibody (mAb), was covalently coupled to the surface of 5-Fluorouracil (5-FU) loaded bovine serum albumin (BSA) nanoparticles. Concerning two different crosslinkers for conjugation of 1F2, Maleimide-poly (ethylene glycol)-Succinimidyl carbonate (Mal-PEG5000-NHS) was selected due to its higher conjugation efficiency (23±4 %) obtained in comparison to N-succi...

full text

Targeted Delivery of 5-fluorouracil with Monoclonal Antibody Modified Bovine Serum Albumin Nanoparticles

full text

Targeted Delivery of 5-fluorouracil with Monoclonal Antibody Modified Bovine Serum Albumin Nanoparticles

full text

Producing Gelatin Nanoparticles as Delivery System for Bovine Serum Albumin

Background: Progress in the field of biology and biochemistry has led to the discovery of numerous bioactive peptides and proteins in the last few decades. Delivery of therapeutic proteins/peptides has received a considerable amount of attention in recent years. Methods: In this study, a two-step desolvation method was used to produce biodegradable hydrophilic gelatin nanoparticles (GNP) as a d...

full text

Preparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles

Paclitaxel (Taxol(®)) is an important anticancer drug in clinical use for treatment of a variety of cancers. Because of its low solubility, it is formulated in high concentration in Cremophor EL(®) which induces hypersensitivity reactions. In this study, targeted delivery of paclitaxel-loaded nanoparticles was prepared by a desolvation procedure, crosslinked on the wall material of bovine serum...

full text

Producing gelatin nanoparticles as delivery system for bovine serum albumin.

BACKGROUND Progress in the field of biology and biochemistry has led to the discovery of numerous bioactive peptides and proteins in the last few decades. Delivery of therapeutic proteins/peptides has received a considerable amount of attention in recent years. METHODS In this study, a two-step desolvation method was used to produce biodegradable hydrophilic gelatin nanoparticles (GNP) as a d...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}

Journal title:

iranian journal of pharmaceutical research

جلد ۱۴، شماره ۲، صفحات ۳۹۵-۴۰۵

Keywords

[ 5 , ' f l o u r o u r a c i l ' , ' b o v i n e s e r u m a l b u m i n n a n o p a r t i c l e s ' , ' 1 f 2 m o n o c l o n a l a n t i b o d y ' ]

Hosted on Doprax cloud platform doprax.com